Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 3

Percentage of S.  cerevisiae cells deflocculation, yeast precipitation, and turbidity measured in 600 nm by spectrophotometry.

Total turbidity (600 nm)Yeast deflocculation (%)Yeast precipitation

≤12.00p2
27.025.0np3
40.537.5np
54.050.0np
67.562.5np
81.075.0np
94.587.5np
≥120.01100np

1Maximum deflocculation of 30% (w⋅v−1) yeast cell solution by sulfuric acid; 2p = cells precipitated in the bottom of the suspension; 3np = cells suspended in liquid.