Enzyme Research

Research Article

An Efficient and Improved Methodology for the Screening of Industrially Valuable Xylano-Pectino-Cellulolytic Microbes

Table 2

Morphological, physiological, biochemical tests and 16S rDNA sequencing of the isolated microorganism AVS 13.
TestsResultsTestsResults
Morphological tests:
Colony Morphology
ConfigurationCircularMarginEntire
ElevationRaisedSurfaceRough
TextureMucoidPigmentOff-white
OpacityOpaqueGram’s Reaction+
Cell shapeRodSpore(s)+
Motility+  
Physiological tests:
Growth at temperatures
4°C−15°C−
25°C+30°C+
37°C+42°C+
55°C+  
Growth at pH
pH 5.0+pH 6.0+
pH 7.0+pH 8.0+
pH 9.0+pH 10.0+
pH 11.0+pH 12.0+
Growth on NaCl (%)
2.0+4.0+
6.0+8.0+
10.0+11.0+
12.0+  
Biochemical tests:
Growth on MacConkeyNon Lactose FermentingIndole test−
Methyl red test−Voges Proskauer test+
Citrate utilization−Casein hydrolysis+
Esculin hydrolysis+Gelatin hydrolysis+
Starch hydrolysis+Nitrate reduction+
Ornithine decarboxylase+Lysine decarboxylase+
Catalase Test+Oxidase test+
Acid production from
Adonitol−Trehalose+
Rhamnose−Salicin+
Dulcitol−Galactose−
Melibiose+Raffinose−
Inulin+Sorbitol+
Fructose+Sucrose+
16S rDNA SEQUENCING
TGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAAC
ACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATG
GTTGTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACC
CGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACC
TGAGAGGGTGTCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCA
GTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGG
TTTTCGGATCGTAAAGTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACC
TTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTA
GGTGGCAAGCGTTGTCCGGAATATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTG
ATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGA
AGAGGAGAGTGGAATTCCACGTGTAGGGTGAAATGCGTAGAGATGTGGAGGAACACCAGT
GGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACA
GGATTAGATACCCTGGTAGTCCACGCCGTAAAGATGAGTGCTAAGTGTTAGGGGGTTTCCG
CCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGA
AACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGAGCATGTGGTTTAATTCGAAGCA
ACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGAGATAGGACGTCCCC
TTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCCGTGTCGTGAGATGTTGGGT
TAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAG
GTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAACATCATGCCCCTTAT
GACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTA
AGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTCGTGAAGCT
GGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACA
CCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTAGG
+: Positive; −: Negative.