Chitinases from Bacteria to Human: Properties, Applications, and Future Perspectives

Figure 4

A schematic diagram showing methodology of directed laboratory evolution technique for the creation of new enzymes with enhanced catalytic activity. Chitinase gene library is created by random mutagenic PCR, which randomly incorporates desired number of amino acid mutations. Then the PCR product is cloned, expressed, and screened for higher catalytic activity. For detailed methodology, kindly refer to [9, 10].