Figure 1: Time course of lipase production by C. viswanathii in solid-state cultivation using wheat bran plus barley spent grain 1 : 1 (w/w) supplemented with 25% (w/w) olive oil. Cultures were carried out without nitrogen source supplementation and 50% (w/v) initial moisture provided by Vogel salts solution at 28°C for 120 hours. ■: lipase production (U/gds); : specific activity (U/mg of protein).