Figure 7: Activity of crude and purified C. viswanathii lipase on p-nitrophenyl esters. Activity was determined in McIlvaine buffer pH 5.0 at 50°C for crude enzyme and 5.5 at 45°C for purified enzyme. ■: crude lipase; □: purified lipase. C2 acetate, C4 butyrate, C8 caproate, C10 decanoate, C12 laurate, C14 myristate, C16 palmitate, and C18 stearate.