Table 3: Effect of temperature and moisture on lipase production by C. viswanathii in solid-state cultivation.

ParameterLipase activity (U/gds)Specific activity (U/mg prot)

Temperature (°C)
 1526.10 ± 4.4213.31 ± 1.92
 2056.05 ± 6.0722.97 ± 2.43
 2577.71 ± 3.3628.59 ± 2.71
 30143.36 ± 9.6565.76 ± 3.18
 35129.11 ± 8.1543.19 ± 2.98
 4025.80 ± 6.1710.05 ± 1.56

Moisture (%)
 2098.70 ± 4.8778.89 ± 3.47
 30147.55 ± 8.43111.32 ± 4.55
 40157.33 ± 9.25136.20 ± 5.74
 50143.36 ± 7.47113.32 ± 6.40
 60137.56 ± 6.3474.56 ± 5.70
 7092.43 ± 5.7860.80 ± 4.85

Cultures were carried out using wheat bran plus barley spent grain (1 : 1, w/w), 40% (w/w) poultry fat, and 3.5% (w/w) yeast extract for 5 days (above). Cultures were carried out in the same conditions and at 30°C (below).