Table 6: Effect of organic solvents on crude lipase stability produced by C. viswanathii under solid-state cultivation.

Organic solventCrude enzymePurified enzyme
Relative activity (%)Stability (%)Relative activity (%)Stability (%)

Control100.0 ± 1.2100.0 ± 2.0100.0 ± 2.4100.0 ± 3.2
Glycerol−1.67135.4 ± 4.299.5 ± 2.6109.4 ± 5.760.2 ± 2.0
DMSO−1.38111.7 ± 4.099.8 ± 3.0113.5 ± 2.559.5 ± 3.4
Propylene glycol−0.92111.5 ± 3.188.7 ± 3.597.5 ± 7.760.1 ± 3.4
Methanol−0.76105.1 ± 3.384.6 ± 1.9103.2 ± 7.552.9 ± 6.1
Acetonitrile−0.4086.2 ± 1.368.1 ± 3.988.6 ± 8.258.6 ± 2.8
Ethanol−0.24106.2 ± 2.290.5 ± 4.8106.8 ± 6.194.5 ± 2.1
Acetone−0.2390.5 ± 2.266.7 ± 2.485.8 ± 6.869.2 ± 1.8
1-Propanol0.0797.9 ± 2.572.4 ± 1.3111.5 ± 9.966.9 ± 1.8
2-Propanol0.2578.3 ± 2.656.0 ± 2.483.0 ± 4.359.2 ± 1.4
n-Butanol0.8092.0 ± 4.012.1 ± 2.684.1 ± 9.958.3 ± 3.5
Toluene2.5091.1 ± 3.369.8 ± 1.987.2 ± 5.325.0 ± 2.6
Xylol3.1565.9 ± 1.451.5 ± 1.382.0 ± 7.439.1 ± 4.2
n-Hexane3.50105.9 ± 2.598.0 ± 4.798.5 ± 8.665.2 ± 3.1
Isooctane4.5190.4 ± 2.495.1 ± 3.491.4 ± 7.861.8 ± 2.0

Assay conditions: for stability assays crude and purified lipase were incubated at 30°C at 200 rpm during 2 h and the activity was assayed with p-NPP using McIlvaine buffer pH 5.0, at 50°C. : logarithm of the partition coefficient () in octanol/water two-phase system indicates the solvents hydrophobicity. DMSO: dimethyl sulfoxide.