Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility

Figure 1

Schematic of the synthesis of active lysyl oxidase. An inactive propeptide is glycosylated and exported to the extracellular matrix following the incorporation of copper and the formation of the LTQ catalytic cofactor. The propeptide region is cleaved by procollagen C-proteinase at Gly 168 and Asp 169, yielding the propeptide and the 29 kDa active mature enzyme.