Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility

Table 2

Yield, activity, and copper incorporation of each tagged form of lysyl oxidase. The line labeled LOX is data from a previously published paper [15] related to untagged and urea solubilized LOX. This enzyme was not subjected to copper dialysis and hence the lower copper incorporation.

	Protein yield (mg)	Specific activity (U/mg)	Copper incorporation
LOX	10.0	0.31	19%
LOX Nus-A tag	1.5	0.11	68%
LOX Trx tag	7.8	0.03	74%
LOX GST tag	9.3	None Detected	270%

One activity unit is defined as 1 μmol of H₂O₂ produced per minute per mg of enzyme at 37°C.