Table 6: Frequency of common allelic mutations in this study compared with previous studies from some of the neighboring and Arab countries.

Present study, Iraq, 2015Turkey, 2009 [10]Jordan, 2011 [11]Iran, 2011 [12]Iran, 2008 [13]Tunisia, 2004 [14]

Total alleles ()124919288100102
Not detected (%)39.5%15.4%40%030%5.9%
Molecular method(s) usedAS-PCR with reverse hybridization for 11 mutationsSB, AS-PCR/ER, and sequencingARMS for 8 common mutations & MLPAAS-PCR for 8 common mutations and sequencingAS-PCR for 8 mutationsER + sequencing
P30L0017.8000
I2Splice14.522.035.714.772817.6
Del 8 bp E33.24.4160130
I172N8.19.9165.689.010.8
Cluster E602.21.72.274.00
V281L4.07.601.143.00
L307fs00NT0NT0
Q318X11.33.323.215.91935.3
R356W08.83.57.955.02.0
P453S0.812.2NTNRNTNR
R483P0.81NRNTNRNT
Deletions/conversions19.38.8/14.3031.8NT19.6

NR: not reported; NT: not tested; SB: southern blot; AS-PCR: allele-specific oligonucleotide hybridization by PCR; ER: enzyme restriction; ARMS: amplification refractory mutational screen; MLPA: multiplex ligation dependent probe amplification.