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Enzyme Research
Volume 2017, Article ID 4362704, 18 pages
Research Article

Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis

1Biological Science Department, Universidade Estadual Paulista (UNESP), Avenida Dom Antônio, 2100 Bairro, Parque Universitário, 19806-900 Assis, SP, Brazil
2Bioprocess & Biotechnology Department, Universidade Estadual Paulista (UNESP), Rod. Araraquara-Jaú Km 1 Bairro, Machados, 14800-901 Araraquara, SP, Brazil

Correspondence should be addressed to D. F. Silva; moc.liamg@avlisfsalguod

Received 9 September 2016; Revised 25 November 2016; Accepted 14 February 2017; Published 29 March 2017

Academic Editor: Raffaele Porta

Copyright © 2017 D. F. Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced by the shape (drop or sheet) and type of the mixture. The enzyme was recycled 28 times for sheets and 13 times for drops . The derivative showed the highest stability in the recycle obtaining 0.05 FPU/g, RA of 10%, and FPU Yield of 1.64 times, higher than FPU spent or Net FPU Yield of 5.3 times, saving more active enzymes. The derivative B showed stability in recycles reaching 0.15 FPU/g of derivative, yield of Recovered Activity (RA) of 25%, and FPU Yield of 1.57 times, higher than FPU spent on immobilization or Net PFU Yield of 2.81 times. The latex increased stability and resistance of the drops but did not improve the FPU/gram of derivative.