Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
Table 3
Parameters of cellulase immobilized in different derivatives.
Derivatives
IP1
IPY2
FPU/g3
IY5 (%)
RA6 (%)
LA7 (%)
Control-free enzyme (5%)
0.4384
100
100
—
Drops
A (calcium alginate)
11.9
85.6
13.4
96.0
0.53
(calcium alginate + glut.8)
12.0
86.1
13.5
52.2
6.47
B (calcium alginate + chitosan)
12.0
86.3
56.1
25.0
42.1
(calcium alginate + chitosan + glut.)
12.0
86.5
55.4
28.5
39.6
F (calcium alginate)
5.0
48.8
15.4
76.0
3.7
(calcium alginate + glut.)
5.3
52.0
51.5
16.0
43.3
G (calcium alginate + chitosan)
4.9
48.6
13.5
51.4
6.6
(calcium alginate + chitosan + glut.)
5.3
52.4
13.4
55.7
6.0
K (calcium alginate + chitosan + 3% latex)
1.9
84.9
49.37
44.8
27.2
L (calcium alginate + chitosan + 5% latex)
2.0
98.4
42.6
62.2
16.0
M (calcium alginate + chitosan + 10% latex)
2.1
99.5
45.8
73.0
12.3
Sheets
C (calcium alginate)
9.3
94.7
36.3
12.6
31.8
(calcium alginate + glut.)
9.6
97.5
53.5
6.78
49.9
D (calcium alginate + chitosan)
5.9
33.1
22.4
54.1
10.3
(calcium alginate + chitosan + glut.)
5.9
33.1
17.8
8.77
16.2
E (calcium alginate + resin)
10.6
88.8
36.2
14.7
30.9
(calcium alginate + resin + glut.)
11.2
93.8
30.8
10.0
27.7
H (calcium alginate)
2.7
27.9
28.5
15.0
24.2
(calcium alginate + glut.)
3.2
32.4
35.3
10.7
31.5
I (calcium alginate + chitosan)
0.1
1.1
55.4
3.5
53.5
(calcium alginate + chitosan + glut.)
4.5
45.8
46.1
7.9
42.4
J (calcium alginate + resin)
1.4
15.0
23.1
13.2
20.0
(calcium alginate + resin + glut.)
1.6
16.0
24.6
15.2
20.8
Other derivatives
Activated carbon
0.0
0.0
0.0
0.0
100.0
Zeolite
0.46
4.6
83.3
13.4
72.1
Ion exchange resin
0.97
9.7
33.3
81.7
6.1
Polystyrene
2.26
22.7
68.8
20.0
55.0
3% calcium alginate + 5% latex
1.7
33.5
35.0
100.0
—
3% calcium alginate + 0.5% chitosan + 5% latex
2.0
71.5
48.0
49.2
24.9
3% calcium alginate + 1% chitosan + 5% latex
2.0
98.4
42.6
62.2
16.0
1% chitosan + 5% latex9
0.0
0.0
0.0
0.0
0.0
100.0
Amount of immobilized protein (mg protein/gram of support); 2Immobilized Protein Yield (%); 3Enzymatic activity (FPU) per gram of support; 4Enzymatic activity (FPU/mL) of enzyme solution (5%); 5Immobilization Yield (%); 6Recovered Activity (%); 7Lost Activity (%); 8Treatment with 0.5% glutaraldehyde (%); 9there was no solubilization of chitosan in latex. Obs. different letters indicate that they are statistically different ().
