Research Article
Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
Table 5
Number of recycles of celluloses immobilized in pure and hybrid derivatives on reaction with paper filter as substrate.
| | Derivat1 | Glut.2 | Recycle number | Sum of FPU in all recycles (FPU/g) | | Enzyme added during derivative preparation | Enzyme immerged on support | Enzyme added during derivative preparation | Enzyme immerged on support | | Sheet | Drop | Sheet | Drop | Sheet | Drop | Sheet | Drop |
| | Calcium align. | No | 27 (C) | 6 (A) | 4 (H) | 4 (F) | 1.72 | 0.36 | 0.25 | 0.20 | | Yes | 27 () | 7 () | 4 (H) | 4 () | 1.37 | 0.17 | 0.25 | 0.13 | | Calcium align. + chitosan | No | 25 (D) | 13 (B) | 1 (I) | 4 (G) | 0.81 | 1.59 | 0.05 | 0.14 | | Yes | 25 () | 13 () | 5 () | 4 () | 0.74 | 1.62 | 0.43 | 0.12 | | Calcium align. + resin | No | 28 (E) | | 4 (J) | NP | 1.95 | NP | 0.16 | NP | | Yes | 28 () | NP | 5 () | NP | 2.30 | NP | 0.24 | NP | | 3% latex | No | NP | 8 (K) | NP | NP | NP | 0.17 | NP | NP | | 5% latex | No | NP | 6 (L) | NP | NP | NP | 0.42 | NP | NP | | 10% latex | No | NP | 4 (M) | NP | NP | NP | 0.19 | NP | NP |
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The total of endoglucanase supplied to prepare each derivative was , , = 1.10 FPU/g support, , K/L/M – 1.01 FPU/g support; C/, = 1.4 FPU/g support, = 0.98 FPU/g support, = 1.1 FPU/g support, = 1.22 FPU/g support; = 1.81 FPU/g support, J/J′ = 0.868 FPU/g support; 2Treated with 0.5% glutaraldehyde in 1 hour. performed.
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