Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

Table 2

PBED observed and predicted values of factors having an effect on laccase activity.

Tx	Factor type	Culture media volume (mL)	CuSO4; (mM)	Inoculum; (% v/v)	Glucose; (gL^−1)	NH4SO4; (mM)	Peptone; (gL^−1)	Yeast extract (gL^−1)	Observed; enz. activity at 168 h; (UL^−1)	Predicted enz. activity at 168 h (UL^−1)
T1	Factorial	150	1	10	30	5	10	5	4.6928	4.6368
	Factorial	300	0.1	10	30	20	10	5	0.2133	0.1182
	Factorial	300	1	2	30	20	20	5	0.3413	0.3635
	Factorial	150	1	10	10	20	20	10	0.3413	0.2853
	Factorial	300	0.1	10	30	5	20	10	0.2559	0.2782
	Factorial	150	1	2	30	20	10	10	0.9813	1.0426
	Factorial	150	0.1	10	10	20	20	5	0.2133	0.1573
	Factorial	150	0.1	2	30	5	20	10	0.2559	0.1999
	Factorial	300	0.1	2	10	20	10	10	1.9625	1.9847
	Factorial	300	1	2	10	5	20	5	0.1279	0.1502
	Factorial	300	1	10	10	5	10	10	0.0427	−0.0524
	Factorial	150	0.1	2	10	5	10	5	0.2133	0.2746
Central point		225	0.55	6	20	12.5	15	7.5	0.4693	0.7866
Central point		225	0.55	6	20	12.5	15	7.5	1.2372	0.7866
Central point		225	0.55	6	20	12.5	15	7.5	0.9386	0.7866

Best treatments are in bold.