Table 2: PBED observed and predicted values of factors having an effect on laccase activity.

TxFactor typeCulture media volume (mL)CuSO4
(mM)
Inoculum
(% v/v)
Glucose
(gL−1)
NH4SO4
(mM)
Peptone
(gL−1)
Yeast extract (gL−1)Observed
enz. activity at 168 h
(UL−1)
Predicted enz. activity at 168 h (UL−1)

T1Factorial1501103051054.69284.6368
Factorial3000.11030201050.21330.1182
Factorial3001230202050.34130.3635
Factorial150110102020100.34130.2853
Factorial3000.11030520100.25590.2782
Factorial15012302010100.98131.0426
Factorial1500.11010202050.21330.1573
Factorial1500.1230520100.25590.1999
Factorial3000.12102010101.96251.9847
Factorial300121052050.12790.1502
Factorial30011010510100.0427−0.0524
Factorial1500.121051050.21330.2746
Central point2250.5562012.5157.50.46930.7866
Central point2250.5562012.5157.51.23720.7866
Central point2250.5562012.5157.50.93860.7866

Best treatments are in bold.