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Enzyme Research
Volume 2017, Article ID 6980565, 8 pages
https://doi.org/10.1155/2017/6980565
Research Article

Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

1Institute for the Study of Inborn Errors of Metabolism, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia
2Departamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia
3Unidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia
4Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia

Correspondence should be addressed to Carlos J. Alméciga-Díaz; oc.ude.anairevaj@agicemlajc and Balkys Quevedo-Hidalgo; oc.ude.anairevaj@odeveuqb

Received 12 May 2017; Revised 19 July 2017; Accepted 2 August 2017; Published 30 August 2017

Academic Editor: Sunney I. Chan

Copyright © 2017 Dennis J. Díaz-Rincón et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary materials show: (1) Comparison of S. cerevisiae and W. anomalus codon usage, (2) Expression vector pKS2-ST::CBHII, (3) Wickerhamomyces anomalus 54-A identification by ITS amplification and MALDI-TOF, (4) Residual glucose during the culture of W. anomalus 54-A pKS2-ST::CBHII clones, and (5) Estimation of kinetic parameters for the recombinant T. reesei CBHII.

  1. Supplementary Material