Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR)

Figure 3

Double-reciprocal plot of the initial velocity data. Each line was obtained by varying the concentration of both NADPH and GSSG such that [GSSG] = χ [NADPH]. The corresponding χ factor values were as follows: (●) 1; (○) 2.5; (▲) 8. Final concentration of enzyme subunit was 5.3 nM. Lines represent the result of the global adjustment of initial velocity data to the corresponding equation [27]. Each point represents mean ± standard deviation (n=6).