Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR)

<div><b>Dependence of the apparent lag time on the concentration of both NADPH and GSSG</b>. The magnitude of the relaxation time was estimated as described under Materials and Methods from full time courses performed at 25°C and pH 7.8 at the following NADPH concentrations: (Δ) 5.5 <i>μ</i>M; (▲) 9 <i>μ</i>M; (○) 15.5 <i>μ</i>M; (●) 50 <i>μ</i>M. Each point represents mean ± standard deviation (n=6).</div>

Enzyme Research

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Figure 6

Figure 6: Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR) 