Table 1: Recovery of activity fractions of free enzyme after purification of two AChE from A. sexdens using ATCh as substrate.

ProcedureVolumeTotal proteinTotal activitySpecific activityRecovery (%)Purification fold
(mL)(mg)(U)(U/mg protein)

Crude extract10.01915.21340.11001
Ammonium sulfate precipitation2.5888.92410.31804
HiTrap DEAE-FF AsAChE-A2.0279.7660.2493
HiTrap DEAE-FF AsAChE-B3.087.8220.2164

1U: the amount of enzyme that catalyzes the hydrolysis of 1 µM of ATCh per minute.