Acetylcholinesterases from Leaf-Cutting ant Atta sexdens: Purification, Characterization, and Capillary Reactors for On-Flow Assays

Table 1

Recovery of activity fractions of free enzyme after purification of two AChE from A. sexdens using ATCh as substrate.

Procedure	Volume	Total protein	Total activity	Specific activity	Recovery (%)	Purification fold
	(mL)	(mg)	(U)	(U/mg protein)
Crude extract	10.0	1915.2	134	0.1	100	1
Ammonium sulfate precipitation	2.5	888.9	241	0.3	180	4
HiTrap DEAE-FF AsAChE-A	2.0	279.7	66	0.2	49	3
HiTrap DEAE-FF AsAChE-B	3.0	87.8	22	0.2	16	4

1U: the amount of enzyme that catalyzes the hydrolysis of 1 µM of ATCh per minute.