Acetylcholinesterases from Leaf-Cutting ant Atta sexdens: Purification, Characterization, and Capillary Reactors for On-Flow Assays

Table 3

Kinetic parameters of free enzymes toward ATCh and ACh as substrates.

Substrate	Enzyme	Km		Vmax/Km
		(µM)	(µM.min^−1.mL^−1)
ATCh	AsAChE-A	39.1 ± 2.3	23.0 ± 0.4	0.6
	AsAChE-B	52.1 ± 4.7	7.3 ± 0.3	0.1
ACh	AsAChE-A	40.7±12.5	2.3±0.2	0.06
	AsAChE-B	36.0±11.8	1.7±02	0.05