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Enzyme Research
Volume 2019, Article ID 6972835, 27 pages
https://doi.org/10.1155/2019/6972835
Research Article

Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale

1Department of Veterinary Pathobiology, University of Missouri, USA
2Department of Chemistry, University of Missouri, USA

Correspondence should be addressed to Thomas J. Malinski; moc.miehlegni-regnirheob@iksnilam.samoht

Received 25 January 2019; Accepted 6 March 2019; Published 2 May 2019

Academic Editor: Qi-Zhuang Ye

Copyright © 2019 Thomas J. Malinski and Harkewal Singh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. Calculated was 1.1 mM with of 173 s−1. The described enzyme belongs to the GH109 family.