Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale

Figure 11

S. linguale α-NAGA blood group conversion by solution.

(a) Glycine solution (Type O RBC and enzyme treated Type A2 RBC), FITC, and PE fluorescence histograms and scattergram

(b) Alanine solution (Type O RBC and enzyme treated Type A2 RBC), FITC, and PE fluorescence histograms and scattergram

(c) Lysine solution (Type O RBC and enzyme treated Type A2 RBC), FITC, and PE fluorescence histograms and scattergram

(d) Phosphate buffer (Type O RBC and enzyme treated Type A2 RBC) and PE fluorescence histogram (0, 5, 10, 15, 20, and 25 mM NaCl)