(a) 0 – 10 minutes (0, 1, 5, and 10 minutes)
(b) 15 – 60 minutes (15, 30, 45, and 60 minutes and Type O RBC)
(c) 75 – 120 minutes (75, 90, 105, and 120 minutes and Type O RBC)

Figure 21: S. linguale α-NAGA at room temperature (enzyme treated Type A2 RBC) and PE fluorescence histogram (100 μg/mL enzyme).