Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale

Table 2

Temperature Michaelis-Menten steady state kinetics.

Enzyme	Temperature °C	μmole N-Acetylgalactosamine/s	(mM)	s^−1	(s^−1mM^−1)
S. linguale; α-NAGA	20	0.14 ± 0.005	0.63 ± 0.05	128	203
	25	0.19 ± 0.007	1.07 ± 0.07	173	163
	30	0.18 ± 0.012	1.09 ± 0.12	165	152
	35	0.17 ± 0.014	0.94 ± 0.15	154	163
	40	0.14 ± 0.012	0.67 ± 0.12	133	198
	45	0.13 ± 0.006	0.79 ± 0.08	122	154