Research Article

Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale

Table 3

pH Michaelis-Menten steady state kinetics.

EnzymepHµmole N-Acetylgalactosamine/s (mM)s−1 (s−1mM−1)

S. linguale  
α-NAGA
6.00.11 ± 0.010.50 ± 0.08103206
6.40.14 ± 0.020.89 ± 0.18130145
7.00.50 ± 0.050.67 ± 0.12459686
7.40.17 ± 0.010.20 ± 0.04154765
8.00.08 ± 0.010.13 ± 0.0375579