Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
Table 3
Screening of filamentous fungi for lipolytic enzymes production with hydrolytic and esterification activities.
Strains
Biomass (g/L)
Hydrolytic activity (U/mL) 1
Esterification activity (U/mL)
F2
-
-
-
F3
4.25 ± 0.22 i 2
0.05 ± 0.01 f
n.d.
F4
-
-
-
F5
3.79 ± 0.35 j
0.07 ± 0.02 e
0.09 ± 0.04 b
F7
3.89 ± 0.04 j
0.04 ± 0.01 f
n.d.
F8
-
-
-
F11
-
-
-
F14
3.44 ± 0.07 k
0.03 ± 0.00 f
n.d.
F15
3.42 ± 0.03 k
0.02 ± 0.01 f
n.d.
F16
2.40 ± 0.02 l
0.08 ± 0.02 e
n.d.
F20
1.66 ± 0.12 m
0.03 ± 0.003 f
n.d.
F21
0.94 ± 0.06 n
0.01 ± 0.00 f
n.d.
F22
9.19 ± 0.56 d
0.25 ± 0.04 b
0.02 ± 0.01 f
F23
-
-
-
F24
10.76 ± 0.03 a
0.07 ± 0.00 e
n.d.
F25
-
-
-
F26
9.51 ± 0.01 c
0.06 ± 0.00 e
n.d.
F28
-
-
-
F29
-
-
-
F30
7.00 ± 0.15 g
0.07 ± 0.01 e
n.d.
F31
9.40 ± 0.12 c
0.19 ± 0.04 c
0.0
F32
8.91 ± 0.57 e
0.08 ± 0.00 e
n.d.
F33
8.39 ± 0.16 f
0.12 ± 0.02 d
0.06 ± 0.02 d
F35
4.06 ± 0.06 i
0.12 ± 0.01 d
n.d.
F36
8.54 ± 0.06 f
0.19 ± 0.06 c
0.11 ± 0.05 a
F37
8.76 ± 0.02 e
0.19 ± 0.05 c
0.08 ± 0.04 c
F59
-
-
-
F61
5.12 ± 0.04 h
0.62 ± 0.02 a
0.09 ± 0.04 b
F77
-
-
-
F85
-
-
-
F91
-
-
-
F104
8.51 ± 0.06 f
0.18 ± 0.07 c
0.04 ± 0.02 e
F116
9.14 ± 0.03 d
0.06 ± 0.007 e
n.d.
F120
10.14 ± 0.08 b
0.05 ± 0.00 f
n.d.
F125
9.68 ± 0.05 c
0.05 ± 0.01 f
n.d.
F130
9.08 ± 0.09 d
0.07 ± 0.00 e
n.d.
F137
-
-
-
F307
9.68 ± 0.01 c
0.16 ± 0.02 c
0.10 ± 0.04 a
Culture conditions: 1 Submerged cultivation was carried out in Vogel medium with 1.0% olive oil and 0.2% (w/v) yeast extract at 28°C, 180 rpm for 5 days. Label: (+) presence of activity, (-) absence of activity; n.d. no activity detected under the assay conditions. 2 Statistically significant differences between the mean values of hydrolytic activity and esterification were defined using the Scott-Knott test. Averages followed by distinct letters differ by Scott-Knott test at the level of significance 0.05. Pearson correlation was determined using biomass and hydrolytic activity. R-Pearson correlation: 0.16.
