Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
Table 5
Lipolytic enzyme production with hydrolytic and esterification activities in different media composition.
Strains
Media compositions
Hydrolytic activity (U/mL)
(U/g substrate)
Esterification activity (U/mL)
Trichoderma sp. F5
M1
0.71 ± 0.01 b 1
59.64
n.d
M2
0.06 ± 0.01 f
5.04
0.05 ± 0.01 e
M3
0.03 ± 0.00 f
2.40
0.03 ± 0.01 f
M4
0.05 ± 0.00 f
4.00
n.d
M5
0.03 ± 0.00 f
2.52
n.d
M6
0.03 ± 0.00 f
2.52
n.d
Penicillium sp. F36
M1
0.63 ± 0.00 b
52.92
n.d
M2
0.24 ± 0.00 d
22.08
n.d
M3
0.10 ± 0.01 f
8.00
n.d
M4
0.18 ± 0.00 e
15.12
n.d
M5
0.19 ± 0.00 e
17.48
0.04 ± 0.01 f
M6
0.02 ± 0.00 f
1.76
0.09 ± 0.05 b
T. harzianum F37
M1
0.42 ± 0.01 c
36.96
0.03 ± 0.01 f
M2
0.02 ± 0.01 f
1.68
0.08 ± 0.00 c
M3
0.16 ± 0.04 e
15.36
n.d
M4
0.10 ± 0.02 f
8.80
n.d
M5
0.24 ± 0.03 d
20.16
n.d
M6
0.08 ± 0.00 f
7.04
0.05 ± 0.01 e
F. solani F61
M1
0.73 ± 0.01 b
67.16
n.d
M2
0.35 ± 0.01 c
32.2
n.d
M3
2.37 ± 0.01 a
218.04
0.07 ± 0.01 d
M4
0.45 ± 0.02 c
46.06
0.03 ± 0.00 f
M5
0.65 ± 0.01 b
57.20
0.01 ± 0.00 f
M6
0.02 ± 0.00 f
1.84
n.d
T. harzianum F307
M1
0.42 ± 0.01 c
40.32
0.05 ± 0.01 e
M2
0.03 ± 0.00 f
2.88
0.18 ± 0.00 a
M3
0.06 ± 0.00 f
5.76
0.05 ± 0.01 e
M4
0.04 ± 0.00 f
3.52
n.d
M5
0.24 ± 0.01 d
23.04
0.19 ± 0.00 a
M6
0.04 ± 0.00 f
3.36
0.02 ± 0.00 f
Culture conditions: cultivation was carried out with 1.0% olive oil and 0,2% yeast extract, at 28°C and 180 rpm for 5 days. A sample under the same conditions was used as control for each experiment. Label: n.d. no activity detected under the assay conditions. Statistically significant differences between the mean values of biomass, hydrolytic activity, and esterification were defined using the Scott-Knott test. 1averages followed by distinct letters differ by Scott-Knott test at the level of significance 0.05.
