Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
Table 6
Nitrogen sources for lipolytic enzyme production with hydrolytic and esterification activities for F. solani (F61).
Nitrogen sources
Concentration (%)
Biomass (g/L)
Hydrolytic activity (U/mL)
(U/g)
Esterification activity (U/mL)
Organic sources
Corn steep liquor
0.2
5.52 ± 0.01 o 1
0.05 ± 0.01 p
5.25
n.d
1.0
7.54 ± 0.04 j
0.49 ± 0.01 k
44.10
n.d
2.0
9.45 ± 0.04 e
1.10 ± 0.11 h
71.50
n.d
Peptone
0.2
6.86 ± 0.02 l
0.21 ± 0.02 m
22.05
n.d
1.0
8.21 ± 0.02 g
2.15 ± 0.02 f
204.25
0.04 ± 0.01 d
2.0
9.43 ± 0.02 f
1.34 ± 0.14 g
127.30
n.d
Tryptone
0.2
4.45 ± 0.01 p
0.96 ± 0.02 i
91.20
n.d
1.0
10.48 ± 0.02d
2.89 ± 0.01 d
231.30
0.05 ± 0.01 d
2.0
15.19 ± 0.01 a
3.48 ± 0.01 a
278.40
0.02 ± 0.00 e
Urea
0.2
6.54 ± 0.02 n
0.11 ± 0.02 o
11.50
0.01 ± 0.00 e
1.0
7.72 ± 0.02 h
0.41 ± 0.02 l
45.10
n.d
2.0
7.64 ± 0.02 i
0.57 ± 0.04 j
62.70
n.d
Yeast extract
0.2
3.18 ± 0.01 r
2.37 ± 0.02 e
237.00
0.03 ± 0.01 e
1.0
10.88 ± 0.01 c
3.22 ± 0.07 b
241.50
0.03 ± 0.01 e
2.0
14.85 ± 0.01 b
3.14 ± 0.02 c
251.20
n.d
Inorganic sources
Ammonium chloride
0.2
3.54 ± 0.02 q
0.05 ± 0.01 p
5.00
n.d
1.0
0.10 ± 0.01 u
0.10 ± 0.01 o
10.50
0.06 ± 0.01 d
2.0
0.17 ± 0.01 t
0.16 ± 0.02 n
17.60
0.23 ± 0.02 a
Ammonium sulfate
0.2
7.06 ± 0.02 k
0.06 ± 0.01 p
6.00
0.02 ± 0.01 e
1.0
3.01 ± 0.02 s
0.11 ± 0.01 o
12.10
0.10 ± 0.01 c
2.0
6.78 ± 0.01 m
0.21 ± 0.01 m
24.15
0.17 ± 0.01 b
Culture conditions: submerged cultivation was carried out in medium containing g/l - KH2PO4 – 1, MgSO4.H2O – 1.123 e CuSO4 – 0.06. Supplemented with 1.0% olive oil at 28°C, 180 rpm for 5 days. Label: n.d. no activity detected under the assay conditions. 1averages followed by distinct letters differ by Scott-Knott test at the level of significance 0.05. Pearson correlation was determined using biomass and hydrolytic activity. R-Pearson correlation: 0.71.
