Characterization of the Gene Expression Profile of Human Hippocampus in Mesial Temporal Lobe Epilepsy with Hippocampal Sclerosis
Figure 5
P-glycoprotein and MRP-1 expression of brain-derived microvascular endothelial cells (MVECs) of an MTLE and control patient. Morphology of primary hippocampal MVECs after 10 days in culture from an epileptic patient (a). Confluent MVECs exhibit a coblestone morphology with some spindleform-like, elongated cells. Scale bar: 60 μm. P-gp and MRP-1 expression was assessed by flow cytometry of primary hippocampal MVECs isolated either from a patient who underwent selective amygdalohippocampectomy (sAHE) (b) or a control tissue specimen (c). The expression of the endothelial marker endoglin (CD105) was shown to prove the purity of the MVEC cultures (b and c). The open profiles indicate the specific antibodies and the filled profiles the isotype antibodies.
