Table of Contents
Epilepsy Research and Treatment
Volume 2013, Article ID 532657, 6 pages
Research Article

Increased Seizure Latency and Decreased Severity of Pentylenetetrazol-Induced Seizures in Mice after Essential Oil Administration

1Department of Physiology, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece
2Faculty of Agricultural Development, Laboratory of Microbiology, Biotechnology and Hygiene, Orestiada 68200, Greece
3Department of Biochemistry, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece
4Agricultural University of Athens, Department of Science, Laboratory of Chemistry, 75 Iera Odos Str., Athens GR11855, Greece

Received 5 March 2013; Revised 14 May 2013; Accepted 19 May 2013

Academic Editor: Raffaele Manni

Copyright © 2013 Eleni Koutroumanidou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effect of pretreatment with essential oils (EOs) from eight aromatic plants on the seizure latency and severity of pentylenetetrazol- (PTZ-) induced seizures in mice was evaluated. Weight-dependent doses of Rosmarinus officinalis, Ocimum basilicum, Mentha spicata, Mentha pulegium, Lavandula angustifolia, Mentha piperita, Origanum dictamnus, and Origanum vulgare, isolated from the respective aromatic plants from NE Greece, were administered 60 minutes prior to intraperitoneal (i.p.) injection of a lethal dose of PTZ to eight respective groups of Balb-c mice. Control group received only one i.p. PTZ injection. Motor and behavioral activity of the animals after EOs administration, development of tonic-clonic seizures, seizure latency and severity, and percentage of survival after PTZ administration were determined for each group. All groups of mice treated with the EOs showed reduced activity and stability after the administration of the oil, except for those treated with O. vulgare (100% mortality after the administration of the oil). After PTZ administration, mice from the different groups showed increased latency and reduced severity of seizures (ranging from simple twitches to complete seizures). Mice who had received M. piperita demonstrated no seizures and 100% survival. The different drastic component and its concentration could account for the diversity of anticonvulsant effects.