Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations
Figure 6
Incorporations of the cognate or noncognate NMP by the Syn and E. coli TEC18s in the presence of 10 mM Mg2+ or 1 mM Mn2+. (a) The sequence of the nontemplate strand from +1 to +22. Position +19 and the 18-mer transcript are shown in the bold letter and lower case letters, respectively. (b) The products of TEC18 synthesized in the presence of the cognate CTP and the noncognate ATP (0.5 mM each). Reactions were performed in the presence of 10 mM MgCl2 (upper) or 1 mM MnCl2 (lower). The lengths of transcripts are indicated on the both margins. The incorporated nucleotides are also indicated by the arrowheads in the right margin. (c) The amount of transcript involving misincorporation was shown in the percent of the initial amount of TECs. The TECs of the Syn (blue circle) and E. coli (Esc, orange triangle) were incubated with 0.5 mM NTP for the indicated times in the presence of 10 mM MgCl2 (filed symbols) or 1 mM MnCl2 (open symbols).
