Modulation at Age of Onset in Tunisian Huntington Disease Patients: Implication of New Modifier Genes

Table 1

Studied polymorphisms and corresponding oligonucleotide primers.

Genes	Polymorphisms	Primers
GRIN2A	SNP rs890	Forward	5′ TGT ACC CAC ATA TAT ACA GAC AC 3′
		Reverse	5′ TGA GAT GGT AGA TTC CGA CTG A 3′
GRIN2B	SNP rs1969060	Forward	5′ GGA GGG TAG AGC GGA GAA AG 3′
		Reverse	5′ TCC TTT CAC AAG CAG TGT GC 3′
IT15	CAG	Forward	5′ ATG AAG GCC TTC GAG TCC CTC AAG TCC TTC 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG TGC TGC TGC TGC TGC 3′
TCERG1	Gln-Ala	Forward	5′ CAG AAC TGA CAC CTA TGC TTG C 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG CTT GCA CTT GTG CCT GGA C 3′
TBP	CAG	Forward	5′ GAC CCC ACA GCC TAT TCA GA 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG TGC TGC TGC TGC TGC 3′
JPH3	CTG	Forward	5′CCC AGG AAT CTC GTC TTT CA 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG AGC AGC AGC AGC AGC 3′
ATXN1	CAG	Forward	5′ TGG AGG CCT ATT CCA CTC TG 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG TGC TGC TGC TGC TGC 3′
DMPK	CTG	Forward	5′ CTC GAA GGG TCC TTG TAG CC 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG AGC AGC AGC AGC AGC 3′
DRPLA	CAG	Forward	5′ CAC CCA CCAGTC TCA ACA CA 3′
		Reverse	5′TCA GCA TCC CAG TTT GAG ACG TGC TGC TGC TGC TGC 3′
Fluorescent primer*			5′ FAM-TCA GCA TCC CAG TTT GAG ACG 3

The fluorescent primer is identical to 5′ half (underlined) of reverse primers of all triplet repeat polymorphisms. It was added to the PCR mixture in combination with each pair of triplet repeat nucleotide primers.