A New Diagnostic Way for Behcet's Disease: Skin Prick with Self-Saliva

Figure 6

Bes-1 gene expression in the mucocutaneous lesions of patients with Behcet’s disease (BD) [43]. (a) Three of 11 BD patients were positive for Bes-1 DNA in the lesions including aphthous and genital ulcerations and erythema nodosum (EN)-like eruption by amplified polymerase chain reaction (PCR) using the primers: Bes-1-1 (5′-TAATAACCCTGACCAAGCCTA-3′) and Bes-1-2 (5′-CCCTTTCAAAAGTCATAAATC-3′) encoding S. sanguinis. (b) In these positive lesions, Bes-1 DNA was also detected in the cytoplasm of monocytes adhering to the vascular walls and infiltrated around the vessels by PCR in situ hybridization.