Frequency Distribution of Mannose Binding Lectin-2 and Vitamin D Receptor Gene Variants: Putative Markers for Tuberculosis

Table 1

Primers and annealing temperature used in genotyping MBL-2 and VDR polymorphism.

Gene	Specificity	Primers	Amplicon size	Annealing temperature
MBL-2 Preamp	Promoter	5′-AGAGAGGTATTTAGCACTCTGCCAG-3′; 5′-AGGACATCAGTCTCCTCATATCCC-3′	964 bp	60°C
VDR FokI	Exon 2	5′-AGCTGGCCCTGGCACTGACTCT-3′; 5′-GGAAACACCTTGCTTCTTCTCCCTC-3′	265 bp	60°C
VDR BsmI	Intron 8	5′-AAATACCTACTTTGCTGGTTTGCAGA-3′; 5′-CCCAAGGTCACAATAACTTCCTCT-3′	388 bp	60°C
VDR ApaI	Intron 8	5′-TGGACAGAGCATGGACAGGGAG-3′; 5′-CTCTCGGCTACTCTCGGTGATCC-3′	1391 bp	65°C
VDR TaqI	Exon 9	5′-TGGACAGAGCATGGACAGGGAG-3′; 5′-TTAGCTTCATGCTGCACTCAGGC-3′	485 bp	66°C