Frequency Distribution of Mannose Binding Lectin-2 and Vitamin D Receptor Gene Variants: Putative Markers for Tuberculosis

Table 2

The restriction digestion reaction conditions and genotype assignment after the digestion.

SNP	Incubation Temperature	Homozygous wild type	Heterozygote	Homozygous mutant	% agarose gel
FokI	37°C	265 bp	265 bp, 195 bp, 70 bp	195 bp, 70 bp	2%
BsmI	37°C	160 bp, 235 bp	388 bp, 160 bp, 235 bp	388 bp	2%
TaqI	65°C	485 bp	485 bp, 297 bp, 188 bp	297 bp, 188 bp	2%
ApaI	37°C	1174 bp, 217 bp	1391 bp, 1174 bp, 217 bp	1391 bp	1.5%