Assessment of Functional EST-SSR Markers (Sugarcane) in Cross-Species Transferability, Genetic Diversity among Poaceae Plants, and Bulk Segregation Analysis

Table 2

Details of cross transferability of 42 EST-SSR markers in twenty genotypes belonging to cereals and Saccharum related genera and Saccharum species and their cultivars. Lanes 1 to 20 represent number of bands produced in wheat, maize, barley, rice, pearl millet, oat, Sorghum, Narenga, Sclerostachya, Erianthus, Miscanthus, Bandjermasin Hitam, Gunjera, 51NG56, N58, CoS 92423, CoS 88230, UP 9530, CoS 91230, and CoS 8436, respectively.

S. number/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	Primer; polymorphism (%)
SY 28	4	5	6	1	2	3	0	2	5	5	3	2	3	2	3	3	1	3	2	3	95
SY 29	6	3	5	9	3	12	3	5	12	6	7	7	8	7	7	6	7	6	6	8	100
SY 30	1	0	4	0	0	7	0	5	6	6	3	3	4	3	2	3	2	3	2	3	80
SY 31	0	0	0	0	0	0	0	2	3	2	1	1	2	2	2	0	2	2	0	0	50
SY 32	1	0	0	1	0	2	0	2	4	3	1	3	0	0	1	0	0	0	0	0	55
SY 33	0	0	2	2	3	5	3	7	6	4	6	5	5	5	2	3	0	2	3	3	85
SY 34	0	0	0	0	0	0	0	0	0	0	0	3	2	5	4	5	5	5	9	9	85
SY 35	0	0	0	1	0	4	0	5	2	1	0	0	1	1	1	2	2	1	2	1	65
SY 36	0	0	0	0	0	0	0	0	3	0	0	5	2	2	2	0	2	0	0	2	35
SY 37	9	7	5	6	6	5	5	9	12	10	11	8	8	8	8	6	7	6	5	6	100
SY 38	0	0	0	3	0	2	0	7	8	1	0	0	2	2	1	2	0	0	3	0	50
SY 45	0	0	0	0	0	2	0	5	1	0	1	2	1	1	0	0	0	0	0	0	35
SY 46	0	1	4	4	1	0	0	4	4	0	1	0	0	0	0	0	0	0	0	0	35
SY 47	0	1	3	3	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	25
SY 48	0	0	0	0	5	3	0	8	12	0	5	3	3	2	1	3	2	2	0	0	60
SY 53	0	0	0	1	1	3	3	12	9	2	1	1	2	2	1	3	2	1	3	1	85
SY 58	0	0	0	1	1	0	3	2	1	0	0	0	0	0	0	0	0	0	0	0	25
SY 59	0	0	0	0	0	0	0	4	0	3	3	0	0	1	0	0	0	0	0	0	20
SY 61	0	3	7	5	4	0	3	4	5	6	3	4	1	5	3	5	5	5	5	5	90
SY 62	0	0	0	1	0	2	0	4	5	3	2	2	0	0	0	0	0	0	0	0	35
SY 63	0	0	0	0	0	0	0	1	1	4	1	6	1	1	1	0	1	0	2	1	55
SY 65	0	0	0	0	0	0	0	0	0	1	1	1	0	1	2	3	2	2	2	1	50
SY 66	0	0	0	0	0	0	0	1	0	0	0	3	1	2	0	0	3	2	2	1	40
SY 67	1	2	5	6	3	3	0	2	5	6	3	2	0	0	2	0	0	0	0	0	60
SY 69	1	5	2	0	1	0	0	3	1	2	1	1	1	1	1	2	1	0	3	2	80
SY 70	1	0	0	1	0	1	0	5	2	0	0	1	0	0	2	0	3	3	0	0	45
SY 72	1	0	2	0	0	0	0	1	4	5	0	8	5	8	1	3	6	4	6	8	70
SY 73	0	0	2	0	1	2	0	2	2	0	1	3	1	4	1	0	3	0	0	3	60
SY 74	2	1	1	0	1	1	3	1	3	2	1	4	1	5	1	0	3	0	0	2	80
SY 75	1	0	2	3	3	0	0	0	6	3	9	7	6	7	6	0	4	6	0	0	65
SY 76	0	0	0	0	0	2	0	1	2	1	4	0	0	0	1	0	0	0	0	0	30
SY 77	2	5	6	6	8	4	3	9	6	9	3	0	4	4	3	4	3	0	2	0	85
SY 78	2	2	5	6	4	6	4	5	7	6	4	10	9	10	3	2	8	7	5	9	100
SY 79	0	0	0	0	1	0	0	3	5	2	0	5	1	7	7	2	1	0	0	6	55
SY 80	1	1	0	4	0	1	0	4	5	6	2	5	4	5	5	3	5	4	5	5	85
SY 81	0	0	0	1	2	6	0	6	6	8	7	3	3	4	3	3	3	3	3	2	80
SY 82	0	0	10	3	1	4	3	9	1	1	6	5	4	3	5	3	4	4	3	2	90
SY 83	1	2	0	6	2	3	4	2	7	2	1	3	2	3	1	3	3	2	3	3	95
SY 84	0	0	0	1	2	3	0	0	2	0	0	3	3	1	0	3	3	3	3	3	60
SY 86	1	5	6	3	2	5	4	5	8	11	6	5	3	11	5	12	7	6	7	10	100
SY 89	9	6	5	4	1	7	3	7	4	3	10	4	4	5	7	3	4	7	3	5	100
SY 90	5	0	3	2	5	2	3	4	3	4	0	4	3	6	4	4	2	3	6	4	90
Average of transferability	27.22	27.22	47.22	46.67	36.11	55.56	26.11	88.33	98.89	71.11	60.0	73.33	55.56	75.56	55.0	50.56	58.89	51.11	52.78	60.0