Unique AGG Interruption in the CGG Repeats of the FMR1 Gene Exclusively Found in Asians Linked to a Specific SNP Haplotype

Table 1

The oligonucleotide sequences of primers and the PCR conditions.

Locus	Name	Sequence (5′ to 3′)	References	Conc. (μM)	MgCl2 Conc. (mM)	Adjuvant (Conc.)	Annealing temperature (°C)	Product size (bp.)
WEX44; (rs1868140)	WEX44F; WEX44R; WEX44TF; WEX44GR	CTATCTGGGGGCAAATGAACCATAG; CTCTGAGTTTACGCTCCCCAG; CCTAACAGTATAGACCATGATGGAAACATAT; CAGAGAATAGTTTCAGTTTCTCAGTTTAAACTC	This study; This study; Ennis et al. (2007) [12]; Ennis et al. (2007) [12]	0.1; 0.1; 1.5; 1.5	1.5	Q-solution; (1x)	57	Control (333); T allele (134); G allele (262)
WEX82; (rs5904648)	WEX82F; WEX82R; WEX82CF; WEX82TR	GACAACCCATAATCTGTCATTGG; CCACCAGTACTTCCTAATGATA; TATAACCATGTAAAAAGATCTTCAATC; CCTCTGATTATTAATTTATTAATGCA	This study; This study; This study; This study	0.08; 0.08; 3; 7.84	2.5	Betaine; (0.6 M)	62	Control (398); C allele (152); T allele (295)
WEX5; (rs1805420)	WEX5F; WEX5R; WEX5CF; WEX5GR	GAATGTGGCCCTAGATCCAC; GTGCTAACGAGAAATCGGTG; CTTATCACAGCTGCAACTACAC; CAAATTGTCAGACAAGTAAACC	This study; This study; This study; This study	0.1; 0.1; 1; 3	1.25	BSA; (0.13 mg/mL)	60	Control (361); C allele (143); G allele (261)
ATL1; (rs4949)	ATL1F; ATL1R; ATL1AF; ATL1GR2	ACCCTGATGAAGAACTTGTATCTCT; GAAATTACACACATAGGTGGCACT; TGTACATTTTCCAAATGCAAAGA; AGAGACACAGAATCATAAATGC	Brightwell et al. (2002) [13]; Modified	0.1; 0.1; 3; 0.1	1.5	BSA; (0.13 mg/mL)	53	Control (302); A allele (107); G allele (239)
rs25731	731F; 731R	AGATTCCCACCTCCTGTAGG; CATGCTCTGAGTACTGCTC	This study; This study	0.25; 0.25	1.5	—	60	Product (269); Cut by DraI; A allele (125, 119, 25); T allele (150, 119)
IVS10; (rs25714)	IVS10F; IVS10R; IVS10TF; IVS10CR	AAAGCTGATTCAGGAGATTGTG; ACTGCATTAGAGGACAGAGA; CAAGAAGAGGTATGTTACAGTAT; TTATTATATGTGCCACAAAATATTGG	This study; Xu et al. (1999) [14]; This study; This study	0.1; 0.1; 3; 3	1.5	BSA; (0.13 mg/mL)	53	Control (268); T allele (189); C allele (127)
rs25702	702F; 702R; 702AF; 702GR	ACTCAGTTTAGGCAATCCTG; CACAGCTAGTTCATTTGCTG; TCAGTTTAGTTAGTGTGATGTA; GAAATTTTAAGGAGGCATAATC	This study; This study; This study; This study	0.15; 0.15; 3; 2.8	1.5	BSA; (0.13 mg/mL)	55	Control (379); A allele (148); G allele (274)
rs25723	723F; 723R; 723AF; 723CR	GAGCGAGACTGTCTGGGAA; TGGAAGGACTGGAATCCTAG; ACATTTAAAACACATGCACATATA; TTTCAAAGTATGTTTAAGTAGTAG	This study; This study; This study; This study	0.05; 0.05; 0.6; 4	1.5	BSA; (0.13 mg/mL)	55	Control (330); A allele (263); C allele (114)