Research Article

Prevalence of Some Genetic Risk Factors for Nicotine Dependence in Ukraine

Table 1

Primer sequences used for genotyping the selected loci.

LocalizationGeneSNP, allele1/allele2Genotyping method

4p12GABRA2rs279858, A/GAllele-specific PCR. Primers: 5′-TGGAATTTTACCGTGTCTTGTGTC-3′, 5′-ACAGCTAGATTGGCTGGTTGTT-3′, 5′-ATATTATGAGCTACTGATTTT-3′, 5′-ATATTATGAGCTACTGATTTC-3′

5p15.33DAT1rs28363170 (VNTR), 3-16 repeatsAFLP-PCR. Primers: 5′- TGCGGTGTAGGGAACGGCCTGAG-3′, 5′-CTTCCTGGAGGTCACGGCTCAAGG-3′

11p14.1BDNFrs6265, A/GAllele-specific PCR, according to [7]

11q23.2DRD2rs1800497, C/TPCR-RFLP. Primers: 5′-GGCTTAGAACCACCCAGAGT-3′, 5′-GAGCACCTTCCTGAGTGTCA-3′ Restriction endonuclease Taq I

13q14.2HTR2Ars6313, C/TAllele-specific PCR. Primers: 5′-TACAGTAATGACTTTAACTCC-3′, 5′-TACAGTAATGACTTTAACTCT-3′, 5′-AAGGAGAGACACGACGGTGA-3′

15q25.1CHRNA5rs16969968, A/GAllele-specific PCR. Primers: 5′-TGGGGGAAGTGGAGAAGTGA-3′, 5′-ACATTGGAAGCTGCGCTCA-3′, 5′-ACATTGGAAGCTGCGCTCG-3′

19q13.2CYP2A6rs4105144, C/TAllele-specific PCR. Primers: 5′-CAGATATCAATCGCTCTAATCCT-3′, 5′-CAGATATCAATCGCTCTAATCCC-3′, 5′-TTCCTAGTGCCAACCCCAGT-3′

20q11.21DNMT3Brs910083, A/CPCR-RFLP. Primers: 5′-GGAGACTAGAACATTCCCTGTGT-3′, 5′-GGCCATTCAGGGATGCTAATC-3′ Restriction endonuclease Sal I

22q11.21COMTrs4680, A/GReal-time allele-specific PCR with the use of genotyping kit produced by LLC scientific and production company “LYTECH”