Interleukin-16 Gene Polymorphisms Are Considerable Host Genetic Factors for Patients’ Susceptibility to Chronic Hepatitis B Infection

Table 1

PCR and RFLP information for the SNPs of interleukin-16 gene.

Polymorphism	Primer	PCR (bp)	Restriction enzyme (incubation temp °C)	Restricted fragments’ size (bp)
rs4072111; C/T	F: 5′-CACTGTGATCCCGGTCCAGTC-3′; R: 5′-TTCAGGTACAAACCCAGCCAG C-3′	164	BsmAI (55)	C: 164; T: 140 + 24
rs11556218; T/G	F: 5′-GCTCAGGTTCACAGAGTGTTTCCATA-3′; R: 5′-TGTGACAATCACAGCTTGCCTG-3′	171	Nde I (37)	T: 171; G: 147 + 24
rs4778889; T/C	F: 5′-CTCCACACTCAAAGCCTTTTGTTCCTAT A-3′; R: 5′-CCATGTCAAAACGGTAGCCTCAAGC-3′	280	AhdI (37)	T: 280; C: 246 + 34

The underlined base in the forward primer is different from that of the original sequence and serves as the introduction of a recognition site for the restriction enzyme AhdI.