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HPB Surgery
Volume 2009 (2009), Article ID 835965, 8 pages
Research Article

Influence of Portosystemic Shunt on Liver Regeneration after Hepatic Resection in Pigs

1Department of General, Visceral and Transplant Surgery, University Hospital Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, Germany
2Department of General and Transplant Surgery, University Hospital Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria
3Department of Pathology, Academic Teaching Hospital, Carinagasse 47, 6800 Feldkirch, Austria

Received 5 January 2009; Revised 18 June 2009; Accepted 13 July 2009

Academic Editor: Guntars Pupelis

Copyright © 2009 R. Ladurner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The minimal amount of liver mass necessary for regeneration is still a matter of debate. The aim of the study was to analyze liver regeneration factors after extended resection with or without portosystemic shunt. Methods. An extended left hemihepatectomy was performed in 25 domestic pigs, in 15 cases after a portosystemic H-shunt. The expression of Ki-67, VEGF, TGF- ๐›ผ , FGF, and CK-7 was analyzed in paraffin-embedded tissue sections. Results. The volume of the remnant liver increased about 2.5-fold at the end of the first week after resection. With 19 cells/10 Glisson fields versus 4/10, Ki-67-expression was significantly higher in the H-shunt group. VEGF- and CK-7-expressions were significantly higher in the control group. No significant change was found in FGF-expression. The expression of TGF- ๐›ผ was higher, but not significantly, in the control group. Conclusions. The expression of Ki-67, and therefore hepatocyte regeneration, was increased in the shunt group. The expression of CK-7 on biliary epithelium and the expression of VEGF, however, were stronger in the control group.