HPB Surgery

HPB Surgery / 2012 / Article / Fig 2

Research Article

Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation

Figure 2

Light microscopy of livers after aortic clamping. In (a, b, c), the aortas of anesthetized rats were clamped for 0, 60, and 90 min, respectively, and their livers were then infused with cold UW solution containing trypan blue to label nonviable cells, as described in section 2. In d, a liver was first subjected to 10 min of ischemia followed by 5 min of reperfusion before 60 min aortic clamping. Trypan blue was then infused, as described for (a–c). Blue nuclei in eosin-counterstained sections represent mostly nonviable parenchymal cells. Arrows identify examples of nonviable nonparenchymal cells.
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