Minocycline Decreases Liver Injury after Hemorrhagic Shock and Resuscitation in Mice
Figure 6
Minocycline decreases mitochondrial depolarization after hemorrhage and resuscitation. H/R was performed, as described in Figure 1, and intravital multiphoton microscopy of rhodamine 123 fluorescence was performed 4 h after sham operation (a) or resuscitation with vehicle, minocycline, or tetracycline (b–d), as described in materials and methods. Punctate staining of rhodamine 123 denoted polarization of individual mitochondria, whereas dim diffuse cellular staining indicated mitochondrial depolarization. In (e), the average percentage of hepatocytes with depolarized mitochondria is plotted for each H/R treatment group. Mitochondrial depolarization in sham-operated livers was virtually zero and not plotted. Size of individual groups was 5. Bar is 30 μm. * versus vehicle.
