Small-for-Size Liver Transplantation Increases Pulmonary Injury in Rats: Prevention by NIM811

Figure 2

Time course of pulmonary histopathological changes after transplantation of small-for-size liver grafts. In (a)–(d), lungs were harvested at 5, 18, and 38 h after transplantation of QSG. Lung sections were stained with H&E. In (e)–(h), representative images of ED1 staining (𝑛=4 per group) are shown. In (i), alveolar septal thickness was quantified by image analysis of 5 random alveolar septa per image and 10 random images per slide using IPlab 3.7v software. Relative alveolar septal thickness was expressed as the ratio between the thicknesses of different transplantation groups to the sham-operation group. In (j) and (k), ED1 (j) and myeloperoxidase (MPO, (k)) positive cells per high power field (hpf) were counted in 10 random fields using a 40x objective lens in a blinded manner. Values are means ± S.E.M. Group sizes are 4 per group: * 𝑃<0.05 and ** 𝑃<0.01 versus 0 h.