Table of Contents
International Journal of Bacteriology
Volume 2013 (2013), Article ID 127179, 7 pages
http://dx.doi.org/10.1155/2013/127179
Research Article

Survival and Growth of Vibrio cholerae, Escherichia coli, and Salmonella Spp. in Well Water Used for Drinking Purposes in Garoua (North Cameroon)

1University of Maroua, Higher Teachers’ Training College, P.O. Box 55, Maroua, Cameroon
2University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon
3Centre Pasteur du Cameroun, Annexe de Garoua, B.P. 921, Garoua, Cameroon

Received 8 April 2013; Revised 25 June 2013; Accepted 5 July 2013

Academic Editor: Rodrigo E. Mendes

Copyright © 2013 Moussa Djaouda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The ability of strains of faecal bacteria (Vibrio cholerae, Escherichia coli ATCC 25922, and four strains of Salmonella isolated, resp., from well water, pig, poultry, and human urine in Garoua) to survive or grow in well water microcosms was compared. Water samples were obtained from two wells in Garoua (north Cameroun). Autoclaving at 121°C for 15 min and filtration through 0.2 µm filter were used to make microcosms. Microcosms were constituted of unfiltered-autoclaved, filtered-nonautoclaved, and filtered-autoclaved well waters. Bacterial strains were inoculated at initial cell concentration of 3 Log10CFU/mL. All strains were able to survive/grow in used microcosms, and a maximal concentration of 5.61 Log10CFU/mL was observed. Survival abilities were strain and microcosm dependent. The declines were more pronounced in filtered-nonautoclaved water than in the other microcosms. E. coli and Salmonella sp. (poultry strain) lowered to undetectable levels (<1 Log10CFU/mL) after two days of water storage. V. cholera decreased over time, but surviving cells persisted for longer period in filtered-nonautoclaved water from well W1 (1.91 Log10CFU/mL) and well W2 (2.09 Log10CFU/mL). Competition for nutrients and/or thermolabile antimicrobial substances synthesized by “ultramicrocells” or by the autochthonous bacteria retained by the filter might affect the bacterial survival.