Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum

Table 1

Primers and probes.

Mycoplasma hominis tuf (set number 793, TIB MolBio, Aldelphia, NJ; 10X concentration)^a
Primers	tuf 193F	5′ AATTGATATGTTTAAAGATGATGAAAGAGA 3′
	tuf 193R	5′ TGTATCAACAGCATCCATTAATTCC 3′
Probes	tuf 193fl^b	5′ GACGTAAGAAGCCTTCTATCAGAATATGGT FITC 3′
	tuf 193iLC610^c	5′ Red610 TGATGGTGACAATGCTCCTATTATTGCTGGTTC PO4 3′
Ureaplasma species ureC (set number 684, TIB MolBio; 10X concentration)^d
	ureC 158F	5′ CCTGCTTCGTTTAATGTATCTG 3′
Primers	ureC 158R	5′ GAAGATCCAATCTTTGAACAAATCGTA 3′
	ureC 158R5	5′ GAAGATCCAATCTTTGAACAAATTGCT 3′
Probes	ureC 158fl^b	5′ AGCAACTGTTAATGCTAAGTCAATAGCGTTTCCTG FITC 3′
	ureC 158iLC610^c	5′ Red610 GCCCCTCAGTCTTCGTGAATCTTAAGACCACAAGC PO4 3′

 ^atuf target corresponds to 66720–66912 of GenBank accession number FP236530.
^bLabeled with fluorescein on 3′ end.
^cLabeled with LC610 on 5′ end and a phosphate on 3′ end.
^dureC target corresponds to 527786–527943 of GenBank accession number CP001184.