Table of Contents
International Journal of Bacteriology
Volume 2013 (2013), Article ID 324136, 5 pages
http://dx.doi.org/10.1155/2013/324136
Research Article

Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients

Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia

Received 29 January 2013; Revised 26 March 2013; Accepted 28 March 2013

Academic Editor: Sam R. Telford

Copyright © 2013 Tan Xue Ting et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pertussis or whooping cough is a highly infectious respiratory disease caused by Bordetella pertussis. In vaccinating countries, infants, adolescents, and adults are relevant patients groups. A total of 707 clinical specimens were received from major hospitals in Malaysia in year 2011. These specimens were cultured on Regan-Lowe charcoal agar and subjected to end-point PCR, which amplified the repetitive insertion sequence IS481 and pertussis toxin promoter gene. Out of these specimens, 275 were positive: 4 by culture only, 6 by both end-point PCR and culture, and 265 by end-point PCR only. The majority of the positive cases were from ≤3 months old patients (77.1%) ( ). There was no significant association between type of samples collected and end-point PCR results ( ). Our study showed that the end-point PCR technique was able to pick up more positive cases compared to culture method.