Expression, Purification, and Functional Characterization of Atypical Xenocin, Its Immunity Protein, and Their Domains from Xenorhabdus nematophila
Figure 1
(a) Domain map of xenocin and immunity protein. T: translocation domain; R: receptor domain; C: catalytic domain of xenocin; Im: immunity domain; Hm: hemolysin domain of immunity protein. Primers position and orientation for cloning of different domains depicted with arrows. (b) Phylogenetic analysis of immunity protein from X. nematophila with similar protein sequences from other bacterial species. E. coli cloacin DF13 (NP_052371); Klebsiella pneumonia (AAF05609); E. coli colicin-E6 (AAA23081); E. coli E3 (P02984); Klebsiella oxytoca (AY578794.1).
