Characterization of Annur and Bedakam Ecotypes of Coconut from Kerala State, India, Using Microsatellite Markers

Table 1

SSR primers, their sequences, banding patterns, and percent polymorphism.

Sl. no.	Primer	Primer sequence (5′–3′)	Number of bands	Number of polymorphic bands	Polymorphism (%)
1	CAC2	AGCTTTTTCATTGCTGGAAT; CCCCTCCAATACATTTTTCC	4	4	100
2	CAC3	GGCTCTCCAGCAGAGGCTTAC; GGGACACCAGAAAAAGCC	3	3	100
3	CAC4	CCCCTATGCATCAAAACAAG; CTCAGTGTCCGTCTTTGTCC	4	4	100
4	CAC6	TGTACATGTTTTTTGCCCAA; CGATGTAGCTACCTTCCCC	3	3	100
5	CAC8	ATCACCCCAATACAAGGACA; AATTCTATGGTCCACCCACA	3	3	100
6	CAC10	GGAACCTCTTTTGGGTCATT; GATGGAAGGTGGTAATGCTG	3	3	100
7	CAC13	GGGTTTTTTAGATCTTCGGC; CTCAACAATCTGAAGCATCG	4	4	100
8	CNZ1	ATGATGATCTCTGGTTAGGCT; AAATGAGGGTTTGGAAGGATT	4	4	100
9	CNZ2	CTCTTCCCATCATATACCAGC; ACTGGGGGGATCTTATCTCTG	4	4	100
10	CNZ3	CATCTTTCATCATTTAGCTCT; AAACCAAAAGCAAGGAGAAGT	4	4	100
11	CNZ4	TATATGGGATGCTTTAGTGGA; CAAATCGACAGACATCCTAAA	4	4	100
12	CNZ5	CTTATCCAAATCGTCACAGAG; AGGAGAAGCCAGGAAAGATTT	4	4	100
13	CNZ6	ATACTCATCATCATACGACGC; CTCCCACAAAATCATGTTATT	4	4	100
14	CNZ10	CCTATTGCACCTAAGCAATTA; AATGATTTTCGAAGAGAGGTC	4	4	100
15	CnCir56	AACCAGAACTTAAATGTCG; TTTGAACTCTTCTATTGGG	4	4	100
16	CnCirH9	CACAATCCTTACATCAAA; TCTCAAGTTCTTACAGCAGT	3	3	100
17	CnCirG4	AGTATAGTCACGCCAGAAAA; AAACCCATAACCAGCAAG	4	4	100
	Total		63	63
	Average		3.70	3.70