Figure 1: Flow cytometric analysis of control and treated U87-MG cell line for Hsp90α detection. Hsp90α antigen is detected with FITC that conjugated secondary antibody upon flow cytometric analysis; (a) control (wild-type U87-MG cells), (b) U87-MG cells treated with 17AAG, and (c) U87-MG cells treated with shRNA targeted against hsp90α. Overlay image of negative (red) with the positive (blue). These flow cytometric readings are typical of 3 such different experiments.