Table 2: Proteins identified by mass spectrophotometry.

ProteinAccession numberMolecular functionBiological processU87-MG-17AAG/U87-MGU87-MG-shhsp90α/U87-MG

Proteins upregulated by inhibition of Hsp90 by 17AAG and shRNA targeted towards hsp90α
HSP70-1 (homosapiens)gi 4529893Chaperone activityProtein metabolism7.81.3
Hexokinase 1, isoform CRA_d (homosapiens)gi 119574708Catalytic activityMetabolism, energy pathways4.51.9
Tumor rejection antigen (gp96) 1 variant (homosapiens)gi 62088648Heat shock protein activityProtein metabolism3.11.7
Vimentin variant 3 (homosapiens)gi 167887751Structural constituent of cytoskeletonCell growth and/or maintenance 21.2
Pyruvate kinase, muscle (homosapiens)gi 127795697Kinase activityMetabolism, energy pathways6.62.9
Vimentin variant 3 (homosapiens)gi 167887751 Structural constituent of cytoskeletonCell growth and/or maintenance 3.91.4
Heat shock 70 kDa protein 8 isoform 2 (homosapiens)gi 24234686Heat shock protein activity Protein metabolism4.21.2
Chain A, Crystal Structure of Aldose Reductase complexed with Dichlorophenylacetic acidgi 119390284Oxidoreductase activity Metabolism, energy pathway2.81.2
Annexin I (homosapiens)gi 4502101Calcium ion bindingCell communication, signal transduction16.81.7
Glyceraldehyde-3-phosphate dehydrogenase (homosapiens)gi 31645Catalytic activityMetabolism, energy pathway81.9
Ubiquitin carboxyl-terminal Esterase L1 (ubiquitin thiolesterase), Isoform CRA_c (homosapiens)gi 119613387Ubiquitin specific protease activityProtein metabolism2.21
Heat shock protein 27 (homosapiens)gi 662841Chaperone activity Protein metabolism2.71.1
Heat shock protein 27 (homosapiens)gi 662841Chaperone activity Protein metabolism2.71.2
Actin related protein 2/3 complex Subunit 2 (homosapiens)gi 5031599Cytoskeletal protein binding Cytoskeleton organization and biogenesis2.91
Heat shock 70 kDa protein 8 isoform 2 variants (homosapiens)gi 62896815Heat shock protein activity Protein metabolism4.51.3

Proteins downregulated by inhibition of Hsp90 by 17AAG and shRNA targeted towards hsp90α
X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70 kDa) (homosapiens)gi 169145198DNA bindingRegulation of nucleobase, nucleoside, nucleotide, and nucleic acid metabolism, DNA repair.−3.7−1.2
Vimentin (homosapiens)gi 62414289Structural constituent of cytoskeletonCell growth and/or maintenance −2.3−3.4
Vimentin (homosapiens)gi 62414289Structural constituent of cytoskeletonCell growth and/or maintenance −2.1−1.3
SERPINE1 mRNA binding protein 1 isoform 1 gi 66346679RNA bindingRegulation of nucleobase, nucleoside, nucleotide, and nucleic acid metabolism.−2.2−1.4
Calumenin isoform a precursor (homosapiens)gi 4502551Calcium ion bindingCell communication, Signal transduction−1.9−2.5
Phosphoglycerate kinase 1 (homosapiens)gi 4505763Catalytic activityMetabolism, Energy pathways−1.2−2
Aldolase A, fructose-bisphosphate, isoform CRA_bgi 119600342Lyase activityMetabolism, Energy pathways−2−1.6
Predicted Glyceraldehyde-3-phosphate Dehydrogenase-like 6 (homosapiens)gi 169208088Catalytic activityMetabolism, Energy pathways−2.5−1.8
Tropomyosin 4-anaplastic lymphoma kinase fusion protein (homosapiens)gi 13274400Structural constituent of cytoskeletonCell communication, Signal transduction−2−1.4
Eukaryotic translation initiation factor 3, subunit 12gi 10801345Translation regulator activityProtein metabolism−2.7−1.6
Transgelin 2 (homosapiens)gi 4507357UnknownUnknown−3−1
Chain A, Cyclophilin B complexed with (d-(Cholinylester)ser8)-Cyclosporingi 1310882UnknownUnknown−3.7−1.8

Proteins differentially expressed by inhibition of Hsp90 by 17AAG and shRNA targeted towards hsp90α
Collagen, type VI, alpha 1 precursor (homosapiens)gi 87196339Extracellular matrix structural constituentCell growth and/or maintenance2.1−1.5
Chain A, structure of human Annexin A2 in the presence Of calcium ionsgi 56967118Calcium ion bindingSignal transduction, Cell communication2.1−1.1
Heat shock protein beta-1 (homosapiens)gi 4504517Chaperone activityProtein metabolism2.3−1.2
Chain A, Human manganese superoxide dismutase mutant Q143ngi 2780818Superoxide dismutase activityCell proliferation, Anti-apoptosis, Cell growth and/or maintenance2.7−1.2
Chain A, crystal structure of Ca2+-bound form of Des3-23alg-2gi 211939086 UnknownApoptosis2.3−1.1
Transgelin 2 (homosapiens)gi 4507357UnknownUnknown2.4−1.1

Note: The lists of identified peptides having ≥2-fold change were searched against MASCOT database for the corresponding proteins. Proteins were categorized according to their change in expression after treatment with 17AAG and shRNA targeting hsp90α in U87-MG glioma cell line. Protein name, accession number, protein molecular function, biological function, and fold change (volume ratio treated/control) as generated by MASCOT and human protein research database are summarized.