Hormones and Sex-Specific Transcription Factors Jointly Control Yolk Protein Synthesis in Musca domestica
Figure 4
RNA analysis of Md-dsx in and strains. (a) Schematic representation of the Md-dsx gene and its splice variants. The male transcript consists of exons 2, 3, m, and 5; and the female transcript contains exons 2, 3, and 4 [15]. Arrows: primers used for amplification of the two Mdsx splice variants. : common primer, : female-specific primer, : male-specific primer. (b) RT-PCR analysis of mRNA samples prepared from wildtype, and males and females. Of each amplification reaction, 5 L were removed after cycle 24, 27, 30, and 39 and analyzed on a gel to estimate transcript abundance. Level of Md-Sxl transcripts served as an internal control for mRNA quality and abundance.
